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Certificate of Analysis

PRODUCT: Adrenomedullin, Human
CATALOG # 22-2-10

LOT NUMBER: L06037A1

SEQUENCE:

Tyr-Arg-Gln-Ser-Met-Asn-Asn-Phe-Gln-Gly-Leu- Arg-Ser-Phe-Gly-Cys-Arg-Phe-Gly-Thr-Cys-Thr-Val-Gln-Lys-Leu- Ala-His-Gln-Ile-Tyr-Gln-Phe-Thr-Asp-Lys-Asp-Lys-Asp-Asn-Val- Ala-Pro-Arg-Ser-Lys-Ile-Ser-Pro-Gln-Gly-Tyr-NH₂

MOLECULAR WEIGHT: 6030.8 amu
MASS SPECTRAL ANALYSIS: Exhibits correct M.W., see attached Mass Spec..
HPLC ANALYSIS: Peptide Purity 98.0%
SOLUBILITY: Dissolve o.5mg peptide in 500 ul HPLC water.
APPEARANCE: White Powder
PEPTIDE CONTENT: 85%
COUNTER ION: Acetate

AMINO ACID ANALYSIS:

CALC. FOUND CALC. FOUND CALC. FOUND

Ala
Arg
Asx
Cys
Glx
Gly
His 2
4
6
2
6
4
1 2.00
4.10
5.57
1.75
5.76
3.89
1.04 Ile
Leu
Lys
Met
phe
Ser 2
2
4
1
4
2
4 1.80
1.98
4.15
0.90
4.29
1.80
3.27 Thr
Trp
Tyr
Val
Other
Other
3

3
2 2.66

2.80
1.91

REMARK:

Not for human use, research purpose only.

Cys(16)-Cys(21): disulfite bridge.

Water Content: 6%

Acetate Content: 5%

QUALITY ASSURANCE BY:__________________ DATE: 06/27/97 Q.C. Dept.