Peptide Synthesis Company
Peptide synthesis is the production of peptides, organic compounds consisting of multiple amino acids linked by peptide bonds, also known as amide bonds. Peptides are synthesized by coupling the carboxyl group or C-terminus of a single amino acid to the-N terminus, or amino group, of another. The three main steps involved in the process are de-protection of the N-terminal amino acid of the peptide, activation and addition of the next amino acid, and lastly, de-protection of the new N-terminal amino acid.
For synthesis techniques, there are two distinct methods, solid-phase and solution-phase:
Liquid- or solution-phase synthesis is the older of the two techniques, but is still widely used in structure modification synthesis, rare intermediates preparation, and peptide/protein ligation and conjugation. In the peptide industry, solution-phase is more cost-efficient for large scale production of shorter chain peptides, such as luteinizing hormone-releasing hormone (LH-RH) analogues.
Solid-phase synthesis is the method that most labs today use. It can incorporate amino acids that do not occur naturally, modify the peptide/protein backbone, and allow for innate mixing of natural peptides that are difficult to express in bacteria. In this method, amino acids attach to polymer beads suspended in a solution to build peptides, which remain attached to beads until cleaved by a reagent such as trifluoroacetic acid. This immobilizes the peptide during the synthesis so that it can be captured during filtration, while liquid-phase reagents and by-products are simply flushed away. The benefits of solid-phase synthesis are that it greatly speeds production of peptides since it is a relatively simple process; it is easier to scale, and it is more suitable than solution-phase synthesis for longer sequences.
The overall quality of the peptide is determined during the synthesis, purification and analysis stages. American Peptide possesses the requisite experience and dexterity to carry out this complex process.