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CUSTOMER SERVICE
 Can I order online? Yes, you can order online by logging on http://www.americanpeptide.com
I have a specific question about my peptide who should I contact? If you have any further question please do not hesitate to contact any of our technical support or
customer service representatives
at 1-800-926-8272(USA/Canada) or 1-408-733-7604 or by e-mail at: customerservice@americanpeptide.com
Are there any recommended procedures when I am about to start working on
my peptide? When preparing the peptide for use, please observe the following steps to maintain peptide quality:
Warm up the peptide to room temperature prior to opening and weighing out portion
of the peptide. The recommended time for warm up is 1 hour.
In a clean environment weigh out desired quantity of peptide quickly.
Store the remaining peptide in freezer, preferably below -200C
within an enclosed case with desiccants.
How long can I store my peptide when it is in solution? It is not recommended to keep excess peptides in solution. The shelf life of peptides in solution is very limited,
especially for sequences containing cysteine, methionine, tryptophan, asparagine, glutamine, and
N-terminal glutamic acid. In general, aliquot the necessary amounts of peptide for a few days and relyophilize remaining portions
for long term storage if necessary.
How should I store my peptide when it is in solution? If storage of peptides in solution is absolutely unavoidable,
use sterile buffers at pH 5-6 and store aliquots at -200C to prolong the storage life of peptides in solution.
SOLUBILIZATION
How do I solubilize my peptide? All solubility information is reported on the Certificate of Analysis received with your
peptide. Please read the instructions carefully before starting. Solubility may very depending on
the nature of the peptide. The most common dissolution process is 1 mg of peptide distilled in
1 ml of sterile water.
Why the solubility may vary from one peptide to the other?
Solubility is often one of the major challenges for researchers who work
with peptides. Each amino acid exhibits its own physical characteristics. Amino
acids such as leucine, isoleucine, and valine are very hydrophobic. Other amino acids like
lysine, histidine, and arginine are hydrophilic. Thus, different peptides will have different solubilities,
based on the content of the peptides.
What should I do if my peptide does not dissolve well?
As a general rule, peptides should first be dissolved in
distilled, preferably sterile water. If solubility still remains a problem,
try the following steps:
Sonication is helpful to dissolve the peptide
Small amounts of dilute(10%) aqueous acetic acid for basic peptides or aqueous ammonia for
acidic peptides may help dissolution of these peptides.
It is also recommended that the peptide be dissolved to the highest
possible concentration, and then diluted with water or buffer to the working concentration.(Note:
The buffer should be added only after the peptide is completely in solution form because salts may cause aggregation)
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Do I need to solubilize my peptide under specific pH?
It will also be stated on your Certificate of Analysis if any specific PH level is requested.
"ACN" on some solubility description, what does it mean?
ACN stands for Acetonitrile. It may be used in assisting dissolution of some peptides.
MY PEPTIDE
What kind of analysis is included in the American Peptide Products?
At American Peptide Company, all peptides come with complete Certificate
of Analysis. Mass Spec, HPLC and Amino Acid Analysis(as a standard for catalog peptides and optional for custom peptides)
How do I know what is the purity of my peptide?
The purity is documented in the Certificate of
Analysis package with your peptide. Included in the Certificate of Analysis is also the HPLC chromatogram.
What is the AMU unit?
AMU stands for Atomic Mass Unit. It is a common unit measure for peptide.
What is the 2% when I have a 98% purity peptide?
2% consists of truncated or deletion sequence.
What is the other 20% if my peptide content is 80%?
Salt and water.
STORAGE
How should I store my lyophilized peptide when I receive it?
You should store your peptide in the freezer of -200C or below
immediately upon arrival.
Do you have any recommendation on the storage procedures?
Peptides are delivered in lyophilized powder form and often hydroscopic.
Absorption of water will decrease stability of the peptide and may reduce overall peptide
content. For best results,please note:
Place in closed dry environment with desiccants.
Upon arrival, always store lyophilized peptide in a freezer at -200C for maximum stability
Avoid using a frost-free freezer. Changes in moisture and temperature may affect peptide stability.
Ambient temperature during shipping does not affect product life and efficacy.
I live abroad and my peptide was out for few days during shipping and customs.
Is it still good for my research?
In the lyophilized form and properly packaged, peptides in general, can stay in
room temperature without being damaged. Please make sure to store in the freezer upon receipt.
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